Keanu Reeves

Neo ("Kid's Story") (voice)

Carrie-Anne Moss

Trinity ("Kid's Story" and "A Detective Story") (voice)

Clayton Watson

The Kid ("Kid's Story") (voice)

Pamela Adlon

Jue ("Final Flight of the Osiris") / Manabu ("Beyond") (voice)

Melinda Clarke

Alexa ("Matriculated") (voice)

John DiMaggio

Crew Man ("Final Flight of the Osiris") / Kaiser ("Program") (voice)

Kevin Michael Richardson

Thadeus ("Final Flight of Osiris") / Cop ("Kid's Story") / Agent #2 ("World Record")

Tom Kenny

Operator ("Final Flight of Osiris")

Rick Gomez

Pilot ("Final Flight of Osiris")

Tara Strong

Crew Woman ("Final Flight of Osiris") / Nurse ("World Record") / Misha ("Beyond")

Bette Ford

Old Woman ("Final Flight of Osiris")

Debi Derryberry

Kid ("The Second Renaissance Part I & II")