Pooya Mostafaie

Bruce Wayne/Batman (voice)

Uma Thurman

Pamela Isley/Poison Ivy (Voice)

Ralph Fiennes

Alfred Pennyworth (Voice)

Grey DeLisle

Vicki Vale and Jane (Voice)

Frank Welker

Guard (Voice)

Maurice LaMarche

Mr. Freeze (Voice)

Fred Tatasciore

Bane (Voice)

John Glover

Dr Jason Woodrue (Voice)